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Name: Leung Kin Man University number: 2009109720 Date of examine: 25-2-2010 BIOL1133: biologic Science Laboratory Course Experiment 4: Expression of adult males secretin receptor concretion protein in E coli. Aim: The suggest of the experiment is to express human secretin receptor fusion protein in E coli by conducting SDS-PAGE gel electrophoresis and estimate the molecular size of that protein. Abstract: In this experiment, the human secretin receptor fusion protein was expressed exploitation the SDS-PAGE technique. The pre-induced and the sample after(prenominal) IPTG induction was use to extract the human secretin receptor protein by victimisation denaturing buffer zone and elution buffer. The molecular weight of the protein foot also be estimated by comparing the bands on the protein ladder. door: Massive production of active protein is usually done by recombinant deoxyribonucleic acid technology. E coli. was employ as vector for launching an d expression of fusion protein. E coli. is a germ-negative bacteria with treble membrane layer and gamin plasmid. In this experiment, the N-terminal extracellular field of view of the human secretin receptor in E coli. was expressed using IPTG inducible system.

Secretin is the key hormone that prudent for the regulation of gastrointestinal ladder by regulating the secretion of bicarbonate and enzyme from the exocrine pancreas. This earth-closet help to neutralize stomachic acid (hydrochloric acid) and hence obligate an optimum pH care for for intestinal digestion. The action of secretin is principally contr olled by high-affinity membrane receptors di! stributed in our body and it is tissue-specific. The N-terminal secret writing division of the extracellular domain of the receptor protein is obtained by conducting polymerase chain answer (PCR) mutagenesis method. This technique was achieved by using specific deoxyribonucleic acid primers and DNA strand as a template to start the PCR reaction. The coding sequence was introduced into the bacterial DNA as a mailman of the coding sequence. Upon IPTG...If you want to posture a full essay, social club it on our website: BestEssayCheap.com

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